My first publication is finally available to the public, and now I can finally say that I am a 100% bona fide published scientist! The article's primary focus is explaining the technique in which we exercise fruit flies and the precise methods we use to study if adult exercise can reduce the negative effects that arise from consuming a high-fat during childhood. The paper is available to all online through the Journal of Visualized Experiments (JoVE). The article includes a classic manuscript describing in thorough detail our methods for exercising the flies along with a video showing the methods conducted live in our lab by none other than Team TreadWheel itself.
Sections of fly guts on the TEM:
In my great quest to conquer all the microscopes in my department's Optical Analysis Facility, I was most excited to try my hand at the TEM. But before I could view the glorious world only revealed through the power of electron imaging, I needed to take my resin-embedded samples, trim them down to the perfect size, and gently fix them to a grid. Sounds simple enough, but alas, science is never as simple as it sounds.
First picture on the fluorescence microscope:
Blood vessel captured on the compound microscope:
Today in class, we took a break from our TEM samples to jump into a little histology work. For class that day, the lovely Dr. Rasco kindly donated some of her old mouse samples to our class to practice our sectioning, fixing, and slide staining. Unfortunately, one of the two men in the class was absent that day, so there was a disproportionate amount of estrogen to testosterone present in the lab. The irony was not lost on us that Dr. Rasco does developmental work, and as a result, most of the samples she had embedded in paraffin were of the testes and ovaries. Well, there were plenty of women in our class ready to show the world why it should be called HERstology instead of HIStology by taking those testes samples and trimming away at them. As the sole representative of his sex, the man took all our jokes in stride and even decided that this might be the only time he could claim he had his own set of ovaries.
First images from the confocal microscope:
We just got the email that our manuscript has been accepted for publication in the Journal of Visualized Experiments (JoVE)! It's really hard to convey in words how thrilled I am at the moment, but let's just say I handled the moment after reading the good news with the upmost decorum and professionalism. Just don't go verifying that with anyone on the third floor of the building, because they might say something about hearing someone shouting and running to their friends across the hall. Either way, I am so excited for my first publication and have learned a great deal about the writing and publishing process from this experience. Next, we'll have a film crew come in from JoVE to film the video component of paper (hence the visualized experiments part of their name). The manuscript might be accepted, but I can tell the work to get this baby published is far from over.
Captured first images on the scanning electron microscope:
Captured my first image on the TEM (or any electron microscope for that matter):