Sections of fly guts on the TEM:
In my great quest to conquer all the microscopes in my department's Optical Analysis Facility, I was most excited to try my hand at the TEM. But before I could view the glorious world only revealed through the power of electron imaging, I needed to take my resin-embedded samples, trim them down to the perfect size, and gently fix them to a grid. Sounds simple enough, but alas, science is never as simple as it sounds.
First picture on the fluorescence microscope:
Blood vessel captured on the compound microscope:
Today in class, we took a break from our TEM samples to jump into a little histology work. For class that day, the lovely Dr. Rasco kindly donated some of her old mouse samples to our class to practice our sectioning, fixing, and slide staining. Unfortunately, one of the two men in the class was absent that day, so there was a disproportionate amount of estrogen to testosterone present in the lab. The irony was not lost on us that Dr. Rasco does developmental work, and as a result, most of the samples she had embedded in paraffin were of the testes and ovaries. Well, there were plenty of women in our class ready to show the world why it should be called HERstology instead of HIStology by taking those testes samples and trimming away at them. As the sole representative of his sex, the man took all our jokes in stride and even decided that this might be the only time he could claim he had his own set of ovaries.
First images from the confocal microscope:
Captured first images on the scanning electron microscope:
Captured my first image on the TEM (or any electron microscope for that matter):